"Hair growth stimulated by allogenic adipose‑derived stem cells supplemented with ATP in a mouse model of dihydrotestosterone‑induced androgenetic alopecia. López Bran et al. Stem Cell Research & Therapy volume 16, Article number: 292 (2025)

  Authors:   E. López Bran, L. Pozo Pérez, P. Tornero Esteban and M. L. González Morales

López Bran et al. Stem Cell Research & Therapy volume 16, Article number: 292 (2025)

Background Androgenetic alopecia (AGA), also known as male or female pattern hair loss, is the most prevalent form of alopecia worldwide. Current treatments are based on hormone drugs, topical vasodilators and hair transplants. Newer options include stem cell therapy targeted at recovering the capacity for hair follicle regeneration. This study examines the effects of intradermally administering allogenic adipose-derived stem cells (ASCs) per se or supplemented with ATP in a mouse model of dihydrotestosterone (DHT)-induced AGA.

Methods Male and female C57BL6-strain mice were treated with DHT to induce AGA and then given injections of treatment solution in a defined area of the depilated back skin, and the same injections three days later. The treatments tested were several concentrations of ASCs combined with two ATP formulations. Photographs of the treated zones were taken on days 7, 10, 14, 17 and 21 and subjected to Image J analysis. On day 21, skin samples were also obtained for histological analysis. The main outcome measure was percentage treated surface area showing hair regrowth on treatment days 17 and 21 expressed as five categories: null, poor, moderate, intense and complete (20, 40, 60, 80 and 100% respectively).

Results The experimental groups found to show the highest number of male individuals with intense/complete hair regrowth on day 21 were those in which mice received low dose ASCs (1 ∙ ­ 106) combined either with liposomal ATP or non-liposomal ATP. Both these groups showed significant differences compared to controls. In females, while low dose ASC treatments and the high dose ASC + liposomal ATP treatment led to no hair regrowth improvement over the control treatment, medium dose ASC (2 × 106) + non-liposomal ATP gave rise to greater regrowth scores.

Conclusions Hair regrowth was improved in all experimental groups in which male mice were administered stem cell solutions supplemented with ATP. In female mice, the highest hair regrowth scores were observed for the medium dose ASC + liposomal ATP treatment.

Keywords Androgenetic alopecia, Mouse model, Adipose derived stem cells, ATP, Hair growth